Allergenicity of partially hydrolyzed whey and casein formulas evaluated by ImmunoCAP inhibition assay and basophil activation test

نویسندگان

چکیده

Background When exclusive breastfeeding is not possible, partially hydrolyzed formula (PHF) often used as a starter for infants. Some children develop allergic symptoms, including anaphylaxis, after the first intake of cow protein. Therefore, tolerability PHF in infants with cow's milk allergy (CMA) important information. Partially whey (PHWF) well characterized, but those containing both and casein are also available. We evaluated characteristics two PHFs, PHF1 PHF2, vitro ex vivo , compared them PHWF, PHWF1. Methods Residual antigenicity β-lactoglobulin (β-LG) formulas was measured using ELISA. The molecular weight profile determined high-pressure liquid chromatography. IgE reactivity allergenic activity were by ImmunoCAP inhibition assay basophil activation test blood from patients CMA, respectively. Results All participants ( n = 10) had casein-specific IgE. β-LG similar to that PHWF1, it slightly higher than PHWF1 casein. However, ability activate basophils. PHF2 lower β-LG, Conclusion These results suggest CMA respectively, degree binding PHFs does necessarily correspond activation.

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ژورنال

عنوان ژورنال: Frontiers in allergy

سال: 2023

ISSN: ['2673-6101']

DOI: https://doi.org/10.3389/falgy.2023.1207924